\n\n
On this dataset, we demonstrate that\nthe commonly used chelating agent EDTA strongly influences peptide\u2013membrane\ninteractions in zwitterionic POPC membranes. More specifically, the first\ndatasheet presents our results from leakage assays on giant unilamellar\nvesicles (GUVs), showing that EDTA suppresses R9-induced membrane\npermeabilization and largely eliminates the characteristic differences between\nR9 and lysine-rich K9 peptides. The suppressing effect is\nconcentration-dependent and independent of fluorescent labelling. 2) The second\ndatasheet presents our results from Fluorescence Cross-correlation Spectroscopy\n(FCCS) to characterize the binding of R9 and K9 peptides to zwitterionic\nmembranes, showing that EDTA suppresses binding of R9 to the membrane, thus\nlowering the efficiency of R9 translocation across zwitterionic membranes.
\n\nFluorescence microscopy to monitor membrane permeabilization was\nperformed using an Olympus FluoView 1000 MPE system equipped with a PicoQuant laser\nscanning module and diode laser excitation at 470 and 640 nm and additional 532\nnm laser. Images were acquired at resolutions of 512 \u00d7 512 or 1024 \u00d7 1024\npixels with dwell times of 4\u20138 \u00b5s. Image analysis was performed using ImageJ,\nand leakage was quantified for individual vesicles using fluorescence intensity\nratios. Each experiment was repeated independently (at least three times), and\naltogether more than 500 vesicles were analyzed per each condition.
\n\nFCCS\nmeasurements were performed using a custom-built setup based on an Olympus IX71\ninverted microscope. This configuration included a 635 nm dichroic mirror\n(Chroma, USA), a water immersion objective (UPLSAPO 60\u00d7, Olympus, Japan), a 3D\nsample scanning stage (PI Mars XYZ NanoPositioner, PI, Germany), and a 50 \u03bcm\npinhole. Fluorescence detection was achieved using two\nemission filters (697/58 nm and 525/50 nm; Chroma, USA) and a pair of single-photon\navalanche diodes (PDM, MPD, Italy). Excitation was provided by two pulsed\nlasers: 470 nm (LDHP-C-470, PicoQuant, Germany) and 635 nm (LDH-D-C-635,\nPicoQuant, Germany), each operating at a repetition rate of 10 MHz in an\nalternating mode. Laser power was kept around 10 \u03bcW for both channels. Each\nsample was measured for a minimum of 2 minutes and a maximum of 5 minutes.
", "type": {"id": "technical-info", "title": {"cs": "Technick\u00e9 informace", "de": "Technische Informationen", "en": "Technical info", "es": "Informaci\u00f3n t\u00e9cnica", "sv": "Teknisk information"}}, "lang": {"id": "ENG", "title": {"cs": "angli\u010dtina", "en": "English"}}}], "creators": [{"person_or_org": {"type": "personal", "name": "Johanovsk\u00e1, Zuzana", "given_name": "Zuzana", "family_name": "Johanovsk\u00e1", "identifiers": [{"identifier": "0009-0007-4170-4214", "scheme": "orcid"}]}, "affiliations": [{"id": "02sat5y74", "name": "Czech Academy of Sciences, Heyrovsky Institute", "identifiers": [{"identifier": "02sat5y74", "scheme": "ror"}, {"identifier": "grid.425073.7", "scheme": "grid"}, {"identifier": "0000 0004 0633 9822", "scheme": "isni"}]}]}, {"person_or_org": {"type": "personal", "name": "Vazdar, Mario", "given_name": "Mario", "family_name": "Vazdar", "identifiers": [{"identifier": "0000-0001-6905-6174", "scheme": "orcid"}]}, "affiliations": [{"id": "05ggn0a85", "name": "University of Chemistry and Technology, Prague", "identifiers": [{"identifier": "05ggn0a85", "scheme": "ror"}, {"identifier": "grid.448072.d", "scheme": "grid"}, {"identifier": "0000 0004 0635 6059", "scheme": "isni"}]}]}, {"person_or_org": {"type": "personal", "name": "\u0160achl, Radek", "given_name": "Radek", "family_name": "\u0160achl", "identifiers": [{"identifier": "0000-0002-0441-3908", "scheme": "orcid"}]}, "affiliations": [{"id": "02sat5y74", "name": "Czech Academy of Sciences, Heyrovsky Institute", "identifiers": [{"identifier": "02sat5y74", "scheme": "ror"}, {"identifier": "grid.425073.7", "scheme": "grid"}, {"identifier": "0000 0004 0633 9822", "scheme": "isni"}]}]}], "dates": [{"date": "2026-05-04", "type": {"id": "Available", "title": {"cs": "Datum zve\u0159ejn\u011bn\u00ed datasetu", "en": "Date Available"}}, "description": "The date the resource is made publicly available. May be a range.\nTo indicate the end of an embargo period, use Available."}, {"date": "2024-01-01", "type": {"id": "CreatedStart", "title": {"cs": "Datum vytvo\u0159en\u00ed datasetu (od)", "en": "Date Created Start"}}, "description": "The date the resource itself was put together; this could refer to a timeframe in ancient history, the start date (of the range) for a final component, e.g., the finalised file with all the data.\nRecommended for discovery."}, {"date": "2026-04-30", "type": {"id": "CreatedEnd", "title": {"cs": "Datum vytvo\u0159en\u00ed datasetu (do)", "en": "Date Created End"}}, "description": "The date the resource itself was put together; this could refer to a timeframe in ancient history, the end date (of the range) for a final component, e.g., the finalised file with all the data.\nRecommended for discovery."}, {"date": "2024-01-01", "type": {"id": "CollectedStart", "title": {"cs": "Datum sb\u011bru dat (do)", "en": "Date Collected Start"}}, "description": "The date range (start date) in which the resource content was collected.\nTo indicate precise or particular timeframes in which research was conducted."}, {"date": "2026-04-30", "type": {"id": "CollectedEnd", "title": {"cs": "Datum sb\u011bru dat (od)", "en": "Date Collected End"}}, "description": "The date range (end date) in which the resource content was collected.\nTo indicate precise or particular timeframes in which research was conducted."}], "subjects": [{"id": "10610", "subject": "Biofyzika"}, {"subject": "membrane permeabilization"}, {"subject": "Cell penetrating peptides"}, {"subject": "fluorescence microscopy"}, {"subject": "GUVs"}], "funding": [{"funder": {"id": "01pv73b02", "name": "Grantov\u00e1 agentura \u010cesk\u00e9 republiky"}, "award": {"number": "GA25-16117S", "title": {"en": "Controlled Transport of Cell-penetrating Peptides across Biological Membranes \u2013 Synergy of Simulations and Single Molecule Fluorescence Experiments"}, "program": "GA\u010cR standard"}}, {"funder": {"id": "037n8p820", "name": "Ministerstvo \u0161kolstv\u00ed, ml\u00e1de\u017ee a t\u011blov\u00fdchovy"}, "award": {"number": "EH22_008/0004558", "title": {"en": "Advanced Multiscale Materials for Key Enabling Technologies"}, "program": "OPJAK"}}], "resource_type": {"id": "c_ddb1", "title": {"cs": "datov\u00e1 sada", "en": "dataset"}}, "languages": [{"id": "ENG", "title": {"cs": "angli\u010dtina", "en": "English"}}], "rights": [{"id": "4-BY", "title": {"cs": "Creative Commons Uve\u010fte p\u016fvod 4.0 Mezin\u00e1rodn\u00ed licence", "en": "Creative Commons Attribution 4.0 International License"}, "description": {"cs": "Umo\u017e\u0148uje ostatn\u00edm rozmno\u017eovat, roz\u0161i\u0159ovat, vystavovat a sd\u011blovat d\u00edlo a z n\u011bj odvozen\u00e1 d\u00edla pouze p\u0159i uveden\u00ed autora. 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