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Heyrovsky Institute Community"}, "access": {"visibility": "public", "members_visibility": "public", "member_policy": "closed", "record_submission_policy": "open", "review_policy": "closed"}, "custom_fields": {"workflow": "community", "allowed_workflows": ["community"]}, "deletion_status": {"is_deleted": false, "status": "P"}, "children": {"allow": false}}]}, "pids": {"doi": {"identifier": "10.48700/bbn7m-ft085", "provider": "datacite", "client": "datacite"}}, "workflow": "community"}, "versions": {"is_latest": true, "index": 1}, "is_published": true, "is_draft": false, "pids": {"doi": {"identifier": "10.48700/datst.741sm-x1528", "provider": "datacite", "client": "datacite"}, "oai": {"identifier": "oai:datarepo.eosc.cz:datst.741sm-x1528", "provider": "oai"}}, "metadata": {"publication_date": "2024-10-19", "title": "Dataset of calcium influx of Jurkat cells on a functionalized coverslip", "publisher": "\u00dastav fyzik\u00e1ln\u00ed chemie J. Heyrovsk\u00e9ho AV \u010cR", "additional_descriptions": [{"description": "<span>This dataset shows the calcium influx of GCaMPfu transfected Jurkat cells interacting with a surface that mimics microvilli and is functionalized with biotinylated anti-human CD3\u03b5 (MEM-57). The cells were imaged using TIRF microscopy and saved in .tif files. The data shows the first contact of the cell to the surface and the calcium dynamics in single cells. The raw data can be analysed quantitatively by examining the fluorescence produced by each cell.</span>", "type": {"id": "abstract", "title": {"cs": "Abstrakt", "en": "Abstract"}}, "lang": {"id": "eng", "title": {"cs": "angli\u010dtina", "en": "English"}}}, {"description": "<span>Cell culture and transfection. Jurkat cells (clone E6-1; ATCC) were grown in RPMI-1640 medium (Fischer Scientific) supplemented with 10% of fetal bovine serum (Fischer Scientific), non-essential amino acids (Fischer Scientific) and 10 mM HEPES under controlled humidity in an incubator at 37 \u00b0C and 5% CO 2 . The cell line was regularly tested for morphology and mycoplasma infection. For the expression of GCaMP6fu and GCaMP6fast, the Jurkat cells were transiently transfected with DNA plasmids using the Neon\u00ae transfection system (Thermo Fisher Scientific). The transfection was performed using 1 \u00b5g of the DNA vector and 300,000 cells in 0.5 ml of prewarmed supplemented RPMI culture medium. The instrument settings were 3 pulses, each 1500 V for 10 ms. The cells were incubated for 16-24h before their use. Single-cell calcium measurement. The coverslips were mounted, and the cells were prepared and transferred to the coverslip for the experiment as previously described for the large-scale calcium measurement. The cells were imaged in a home-built super-resolution wide-field TIRF microscope in thecnical information</span>", "type": {"id": "methods", "title": {"cs": "Metodologie", "en": "Methods"}}, "lang": {"id": "eng", "title": {"cs": "angli\u010dtina", "en": "English"}}}, {"description": "<span>The imaging was performed with TIRFM using the 488nm laser with a 5% power intensity; the emission was filtered using 525/50 filter (Semrock) in the EMCCD camera (iXon DU-897U, Andor). EM gain was set to 100 with an exposure time of 100 ms; single cells were imaged for at least 15 min. The calcium influx was analyzed by the mean fluorescence intensity (MFI) changes of the cells landing on the coverslips as previously described (Franke et al., 2022).</span>", "type": {"id": "technical-info", "title": {"cs": "Technick\u00e1 informace", "en": "Technical Info"}}, "lang": {"id": "eng", "title": {"cs": "angli\u010dtina", "en": "English"}}}], "creators": [{"person_or_org": {"type": "personal", "name": "Gonzalez Navarro, Jose Alfredo", "given_name": "Jose Alfredo", "family_name": "Gonzalez Navarro", "identifiers": [{"identifier": "0000-0003-1662-9092", "scheme": "orcid"}]}, "affiliations": [{"id": "02sat5y74", "name": "Czech Academy of Sciences, Heyrovsky Institute", "identifiers": [{"identifier": "02sat5y74", "scheme": "ror"}, {"identifier": "grid.425073.7", "scheme": "grid"}, {"identifier": "0000 0004 0633 9822", "scheme": "isni"}]}]}], "contributors": [{"person_or_org": {"type": "personal", "name": "Varghese, Beena", "given_name": "Beena", "family_name": "Varghese", "identifiers": [{"identifier": "0000-0001-7693-4182", "scheme": "orcid"}]}, "role": {"id": "relatedperson", "title": {"cs": "Souvisej\u00edc\u00ed osoba", "en": "Related Person"}}, "affiliations": [{"id": "05ggn0a85", "name": "University of Chemistry and Technology, Prague", "identifiers": [{"identifier": "05ggn0a85", "scheme": "ror"}, {"identifier": "grid.448072.d", "scheme": "grid"}, {"identifier": "0000 0004 0635 6059", "scheme": "isni"}]}]}, {"person_or_org": {"type": "personal", "name": "Kovaricek, Petr", "given_name": "Petr", "family_name": "Kovaricek", "identifiers": [{"identifier": "0000-0002-4404-3606", "scheme": "orcid"}]}, "role": {"id": "projectleader", "title": {"cs": "Vedouc\u00ed projektu", "en": "Project Leader"}}, "affiliations": [{"id": "05ggn0a85", "name": "University of Chemistry and Technology, Prague", "identifiers": [{"identifier": "05ggn0a85", "scheme": "ror"}, {"identifier": "grid.448072.d", "scheme": "grid"}, {"identifier": "0000 0004 0635 6059", "scheme": "isni"}]}]}, {"person_or_org": {"type": "personal", "name": "Cebecauer, Marek", "given_name": "Marek", "family_name": "Cebecauer", "identifiers": [{"identifier": "0000-0002-4606-1218", "scheme": "orcid"}]}, "role": {"id": "supervisor", "title": {"cs": "Supervizor", "en": "Supervisor"}}, "affiliations": [{"id": "02sat5y74", "name": "Czech Academy of Sciences, Heyrovsky Institute", "identifiers": [{"identifier": "02sat5y74", "scheme": "ror"}, {"identifier": "grid.425073.7", "scheme": "grid"}, {"identifier": "0000 0004 0633 9822", "scheme": "isni"}]}]}], "dates": [{"date": "2024-10-19", "type": {"id": "available", "title": {"cs": "Datum zve\u0159ejn\u011bn\u00ed", "en": "Date Available"}}, "description": "The date the resource is made publicly available. May be a range.\nTo indicate the end of an embargo period, use Available."}, {"date": "2023-01-01/2024-06-01", "type": {"id": "created", "title": {"cs": "Datum vytvo\u0159en\u00ed", "en": "Date Created"}}, "description": "The date the resource itself was put together; this could refer to a timeframe in ancient history."}, {"date": "2023-01-01/2024-06-01", "type": {"id": "collected", "title": {"cs": "Datum sb\u011bru", "en": "Date Collected"}}, "description": "The date range."}], "subjects": [{"id": "ford:30102", "subject": "Immunology", "scheme": "FORD", "props": {"parents": "ford:30100", "classification_code": "30102"}, "identifiers": [{"identifier": "https://vocabs.ccmm.cz/registry/codelist/SubjectCategory/30000/30100/30102", "scheme": "url"}]}, {"id": "ford:30404", "subject": "Biomaterials (as related to medical implants, devices, sensors)", "scheme": "FORD", "props": {"parents": "ford:30400", "classification_code": "30404"}, "identifiers": [{"identifier": "https://vocabs.ccmm.cz/registry/codelist/SubjectCategory/30000/30400/30404", "scheme": "url"}]}, {"subject": "Anti-human CD3"}, {"subject": "Calcium Influx"}, {"subject": "TIRF-microscopy"}, {"subject": "Jurkat cells"}], "funding": [{"funder": {"id": "01pv73b02", "name": "Grantov\u00e1 agentura \u010cesk\u00e9 republiky"}, "award": {"number": "GM22-11299M", "title": {"en": "Reaction networks at phase interfaces for dynamic self-assembly"}}}], "resource_type": {"id": "dataset", "title": {"cs": "Datov\u00e1 sada", "en": "Dataset"}}, "languages": [{"id": "eng", "title": {"cs": "angli\u010dtina", "en": "English"}}], "related_resources": [{"publication_date": "2024", "title": "Cell surface morphology mimicking nano-bio platform for immune cell stimulation", "identifiers": [{"identifier": "10.1016/j.isci.2024.111033", "scheme": "doi"}], "creators": [{"person_or_org": {"type": "personal", "name": "Varghese, Beena", "given_name": "Beena", "family_name": "Varghese"}}, {"person_or_org": {"type": "personal", "name": "Gonz\u00e1lez-Navarro, Jos\u00e9 Alfredo", "given_name": "Jos\u00e9 Alfredo", "family_name": "Gonz\u00e1lez-Navarro"}}, {"person_or_org": {"type": "personal", "name": "Guerra, Valentino Libero Pio", "given_name": "Valentino Libero Pio", "family_name": "Guerra"}}, {"person_or_org": {"type": "personal", "name": "Faizulina, Margarita", "given_name": "Margarita", "family_name": "Faizulina"}}, {"person_or_org": {"type": "personal", "name": "Artemieva, Daria", "given_name": "Daria", "family_name": "Artemieva"}}, {"person_or_org": {"type": "personal", "name": "Chum, Tom\u00e1\u0161", "given_name": "Tom\u00e1\u0161", "family_name": "Chum"}}, {"person_or_org": {"type": "personal", "name": "Ramakrishna, Tejaswini Rama Bangalore", "given_name": "Tejaswini Rama Bangalore", "family_name": "Ramakrishna"}}, {"person_or_org": {"type": "personal", "name": "Cebecauer, Marek", "given_name": "Marek", "family_name": "Cebecauer"}}, {"person_or_org": {"type": "personal", "name": "Kova\u0159\u00ed\u010dek, Petr", "given_name": "Petr", "family_name": "Kova\u0159\u00ed\u010dek"}}], "resource_type": {"id": "publication-article", "title": {"cs": "\u010cl\u00e1nek v \u010dasopise", "en": "Journal article"}}}], "rights": [{"id": "cc-by-nc-nd-4.0", "title": {"cs": "Creative Commons Uve\u010fte p\u016fvod-Neu\u017e\u00edvejte komer\u010dn\u011b-Nezpracov\u00e1vejte 4.0 Mezin\u00e1rodn\u00ed licence", "en": "Creative Commons Attribution Non Commercial No Derivatives 4.0 International"}, "description": {"cs": "Umo\u017e\u0148uje ostatn\u00edm rozmno\u017eovat, roz\u0161i\u0159ovat, vystavovat a sd\u011blovat pouze d\u00edlo v p\u016fvodn\u00ed podob\u011b, nikoli d\u00edla z n\u011bj odvozen\u00e1, pouze pro nev\u00fdd\u011ble\u010dn\u00e9 \u00fa\u010dely, je nutn\u00e9 uv\u00e9st autora. 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